Dominik Haudenschild - February 12, 2001 at UCD

To 40ml of Yeast Culture Supernatant add

10ml ice-cold 100% TCA (100g TCA brought to 100ml Milli-Q)

Wait 20 minutes on Ice

Centrifuge at > 10,000g for 10 minutes, decant supernatant

Wash twice with ice-cold 50% Acetone or ice-cold 95% Ethanol

Resuspend protein in 1x SDS-PAGE sample buffer, or in PBS.