Dominik Haudenschild, November 2004 at UCDavis
| 20x SB buffer: | |
| 8g | NaOH |
| 45g | Boric Acid |
| to 1L | with MilliQ water |
pH of 1x should be approximately 8.3. Agarose gels can be run at higher voltage than in TBE/TAE (10 V/cm).
Ref:
From Brody JR, Kern SE. Biotechniques. 2004 Feb;36(2):214-6.