1: Pellet 0.5ml of overnight bacterial culture in Eppendorf tube, discard sup.
2: Resuspend completely in 35µl of cold Resuspension Buffer by vortexing
3: Add 66µl Lysis Buffer, mix by inverting tube gently
4: Add 50µl Precipitation Buffer, mix by short vortex,
5: Incubate on ice for 3-5 minutes
6: Centrifuge top speed for 5 minutes
7: Put Supernatant into fresh 1.5ml tube, discard pellet (has bacterial crap)
8: Precipitate plasmid DNA by with 350µl ethanol
9: Pellet DNA by 5-10 minutes top speed in centrifuge
10: Remove supernatant, wash pellet with 75% ethanol, then air-dry pellet
11: Redissolve DNA in 25µl TE with 20µg/ml DNAse-free Pancreatic RNAse
12: Store DNA in -20

DNA is suitable for restriction enzyme digestion

DNA is NOT suitable for automated sequencing or ligations