TCA Precipitation of DNA
Dominik Haudenschild - May 14, 1997 at Genzyme

1. Spot a volume of sample onto membrane and allow liquid to evaporate
2. Wash with Ice Cold 5% TCA/20mM Sodium Pyrophosphate for 10 minutes
- On Vacuum manifold, use 1-2ml per well (96 well format)
- w/o vacuum manifold, wash 4x 2minutes in 200ml beaker
3. Rinse filter in 80% Ethanol and allow to dry
4. Count in Scintillation fluid