• Prehyb membrane in 50µl/cm2 of ExpressHyb at 68°C
If using roller bottle in hyb oven, use at least 12ml

• Boil probe for 5 minutes at 95°C

• Add probe to 5ml Expresshyb

• Hybridize at 68°C for 1 to 2 hours, overnight is OK but background increases

• Rinse several times at room temerature with 2x SSC + 0.05% SDS

• Wash twice 20 minutes at 50°C with 0.1x SSC + 0.1% SDS

• Wrap in Saran wrap and expose to film overnight