Zeller's solution

Dominik Haudenschild from Urs Hofer, November 15, 1993 at CBRC

Purpose: blocking solution for western blots.

100 mM Tris pH 7.5 for 500 ml: 50 ml of 1 M stock

100 mM MgCl2 50 ml of 1 M stock

1% BSA (w/v) 5g

0.5% Tween 20 25 ml of 10% stock (w/v)

5% FBS 25 ml

1% Triton X-100 50 ml of 10% stock (w/v)

Note: Filter solution through 0.45 µm to remove any precipitates from the BSA or FBS, which might cause background on the blots.

The solution can be preserved by freezing in aliquots at -20o C.

Related Solutions:

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