10x Tris Buffered Saline

25mM Tris

Dominik Haudenschild, 10-28-94 at CBRC

To 800ml Milli-Q water add:

NaCl 80g

KCl 2.0g

Tris Base 30.0g

pH to 8.0 with Concentrated HCl

Bring Volume to 1000ml with Milli-Q