1) Resuspend DNA at 1ug/10ul in 1x Mung Bean Nuclease Buffer

2) Add 0.5 to 1.0 units of Mung Bean Nuclease (NEB# 250S) per

ug of DNA

3) Incubate at 30'C for 30 minutes

4) Put reaction on Ice, add NaCl to 0.2M

5) Do Phenol/Phenol-Chloroform/Chloroform extraction

6) Ethanol precipitate DNA

10x Mung Bean Nuclease Buffer is:

500mM Sodium Acetate pH 5.0

300mM NaCl

10mM ZnSO4