Dominik Haudenschild 7-28-93 at CBRC

1) Thaw cells on ice

2) Add 300ul ice cold 0.1M CaCl2

3) Add 10ng to 100ng of DNA (1 to 10 µl of ligation mixture is OK)

4) Incubate on Ice for 30 minutes

5) Heat shock cells at 43.5°C for 45 seconds (No more than 60 sec)

6) Add 1 ml SOC medium, incubate 37' for 30 to 60 minutes

7) Plate onto LB plate with appropriate antibiotics

8) Incubate overnight and screen resistant colonies for insert